Interference of hemoglobin D on measurements of hemoglobin A1c by the high-performance liquid chromatography HA-8160 in 27 patients.
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چکیده
منابع مشابه
Pitfalls of glycated hemoglobin in the glycemic assessment of diabetes patients with hemoglobin louisville: role of serum fructosamine.
Fasting blood glucose measurements were made using the hexokinase method in a Cobas 6000 (Roche Diagnostics, Rotkreuz, Switzerland). Determinations of HbA1c were performed on ethylenediaminetetraacetic acid–anticoagulated blood sample by two methods: high-performance liquid chromatography (HPLC) (HA-8160 in diabetic mode, A. Menarini Diagnostics, Florence, Italy), which detected no abnormal hem...
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SUMMARY: At present, measurment of glycosylated hemoglobin, especially the stable fraction of hemoglobin (HbA1c) is one of the ways to control diabetes because this measurment can demonstrate total glycemia during last 1.2.3 months. Glycosylated hemoglobin is seen in 3 forms: Ala Alb A1c. Among these hemoglobin Alc is quantitively more than other 2 forms and its difference with other hemoglobi...
متن کاملEffects of hemoglobin C, D, E and S traits on measurements of hemoglobin A1c by twelve methods.
BACKGROUND Hemoglobin C, D Punjab, E or S trait can interfere with hemoglobin A1c (HbA1c) results. We assessed whether they affect results obtained with 12 current assay methods. METHODS Hemoglobin AA (HbAA), HbAC, HbAD Punjab, HbAE and HbAS samples were analyzed on one enzymatic, nine ion-exchange HPLC and two Capillary Electrophoresis methods. Trinity ultra(2) boronate affinity HPLC was the...
متن کاملInterference with hemoglobin A(1C) determination by the hemoglobin variant Shelby.
Hemoglobin variant carrier status was found in a 46-year-old African American man following detection of a falsely elevated hemoglobin A1c (HbA1c) by ionexchange high-performance liquid chromatography (HPLC, VARIANT A1c, Bio-Rad Laboratories, Hercules, CA). Additional analysis of the hemoglobin variant using the Beta Thal Short program (Bio-Rad) revealed an unknown peak with a retention time of...
متن کاملQuantitation of hemoglobin A1a+b and hemoglobin A1c by automated "high-performance" liquid chromatography.
The glycosylated hemoglobins A1a+b and A1c have been rapidly and precisely quantitated in 5-micro L samples of human blood hemolysate (approximately 240 micrograms of hemoglobin) by cation-exchange column chromatography. Total chromatographic is 22.0 min. Proportions of Hb A1c range from 3.85 to 6.71% in normal individuals and from 4.23 to 19.90% in diabetic subjects. Within-day variation was 1...
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ورودعنوان ژورنال:
- Journal of diabetes science and technology
دوره 6 5 شماره
صفحات -
تاریخ انتشار 2012